Accueil > Publications > Recherche par années > Années 2000 > 2001

Aubert, Y ; Perrouault, L ; Helene, C ; Giovannangeli, C ; Asseline, U

Synthesis and properties of triple helix-forming oligodeoxyribonucleotides containing 7-chloro-7-deaza-2 ’-deoxyguanosine

Bioorganic & Medicinal Chemistry 9 (6) 1617-1624

par Administrateur - publié le

Abstract :

Multiple incorporations of 7-chloro-7-deaza-2’-deoxyguanosine in place of 2’-deoxyguanosine have been performed into a triple helix-forming oligodeoxyribonucleotide involving a run of six contiguous guanines designed to bind in a parallel orientation relative to the purine strand of the DNA target. The ability of these modified oligodeoxyribonucleotides to form triple helices in a buffer containing monovalent cations was studied by UV-melting curves analysis, gel shift assay and restriction enzyme protection assay. In the presence of Na+, the incorporation of two, three or five modified nucleosides in the third strand has improved the efficacy of formation of the tripler as compared to that formed with the unmodified oligonucleotide.